Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  :10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
Form and how to use : Prednisolone may be more often found in the form of tablets, they are Prednisolone 5 mg, Prednisolone 10 mg, Prednisolone 20 mg and Prednisolone 40 mg.
You should take this Prednisolone 5 mg by mouth and with food it is necessary, because food helps to prevent stomach upset. The dosage and duration of taking this medication depends on medical conditions. Take Prednisolone 1 to 4 times per day.
Taking Prednisolone 5 mg without prescription may lead to nausea, increased sweating, insomnia, dizziness, shortness of breath, vision changes or other eye problems, increased hunger and many others not listed here. Call a doctor if you noticed such or other cases.
Prednisolone Acetate Ophthalmic Suspension USP 1%
FOR TOPICAL OPHTHALMIC USE ONLY.
INGREDIENTS: Each mL contains:
Active: prednisolone acetate %.
Preservative: benzalkonium chloride %.
Inactives: dibasic sodium phosphate, polysorbate 80, edetate disodium, glycerin, citric acid and/or sodium hydroxide (to adjust pH), purified water.
USUAL DOSAGE: Two drops topically in the eye(s) four times daily.
WARNING: Do not touch dropper tip to any surface, as this may contaminate the suspension.
Read enclosed insert.
SHAKE WELL BEFORE USING.
STORAGE: STORE UPRIGHT at 8° - 24°C (46° - 75° F).
Alcon Laboratories, Inc.
Fort Worth, Texas 76134 for
Princeton, NJ 08540
Printed in USA