Steroid sulfatase deficiency genereviews

Restriction enzymes, such as Eco RI, are used in a wide variety of molecular genetics techniques including cloning , DNA screening and deleting sections of DNA in vitro . Restriction enzymes, like Eco RI, that generate sticky ends of DNA are often used to cut DNA prior to ligation , as the sticky ends make the ligation reaction more efficient. Eco RI can exhibit non-site-specific cutting, known as star activity , depending on the conditions present in the reaction. Conditions that can induce star activity when using Eco RI include low salt concentration, high glycerol concentration, excessive amounts of enzyme present in the reaction, high pH and contamination with certain organic solvents. [5]

He was a member of the RAE2001 and RAE2008 Panels, 2009 REF Expert Group Panel, Wellcome Trust Molecules, Genes & Cells Funding Panel (2006-2011), BBSRC Intracellular Signalling Programme Committee and the EPSRC Synthetic and Biological Chemistry College and is currently a member of the Wellcome Trust Peer Review College. He is on editorial boards including the Journal of Medicinal Chemistry, Molecular Cancer Therapeutics, ChemMedChem and is Associate Editor of the Journal of Steroid Biochemistry & Molecular Biology and an Editor of the Biochemical Journal's BJChemBio.

Steroid sulfatase deficiency genereviews

steroid sulfatase deficiency genereviews

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